Starch Physical Properties: Gelatinization, Retrogradation

Anec > Biology Knowledge

Gelatinization and retrogradation are two important physical properties in food processing and storage. Learning about them not only understands starch deeply, but also explains the changes in starchy food in everyday life.

What happens in starch gelatinization?

Although starch resembles instant coffee powder and powdered milk, it does not dissolve quickly when poured into water. Instead, it sinks to container bottom and absorb very little water initially. When heated to a threshold temperature, they swell violently to be a thick paste. This phenomenon is gelatinization that stems from their complex granular structure. Their crystallinity and molecular organization are lost. A hydrophilic colloid is formed as starch disperses in water.

The three stages

The first stage is a process for reversible water absorption. Water preferentially enters the amorphous regions and bond to hydroxyl groups. It is difficult to enter the semi crystalline region where amylopectin is arranged tightly and neatly. A little of water is absorbed and expansion is slightly, so the semi-crystal has not been destroyed. If starch is dried, you will find it returns to its original state.

When it reaches to gelatinization temperature, water molecules not only enter amorphous region but also penetrate the semi-crystalline region. They have enough kinetic energy to break down hydrogen bonds and unwind double helices of amylopectin. Starch granules absorb a great deal of water irreversibly and suddenly burst to 60-100 times their original volume. At this stage, the orderly structure has transitioned to a disordered state, which cannot revert to its original state even upon drying.

The third stage involves the complete collapse of granules. If heating continues, the attractive force no longer restricts thermal motion, and molecules gradually detaches from granules. Finally, a viscous and semi-transparent paste is formed in water. Sometimes, prolonged heat treatment will degrade some starch molecules.

Starch retrogradation: amylose vs amylopectin

It is essentially a transition from a disordered to a relatively ordered state. When starch is heated in water and cooled, the disrupted molecules gradually reassociate into an ordered structure due to hydrogen bond and decreased thermal motion. These structures are similar to native starch, but not identical. They only have a double helix structure at the entangled regions, but do not have a hilum and radial distribution like native starch granules.

Amylose automatically stick together to form a network that provide hydrogel with elasticity and strength against deformation. It promotes the initial stage of starch retrogradation.

Although side chains in amylopectin sometimes hold together by hydrogen bonds, it's difficult for them to form a stretchy web, just as you would have a hard time to build a web via branched sticks. Therefore, their retrogradation of is slower.

Frequently Asked Questions

Applications of Starch Gelatinization and Retrogradation in Cooking

Gelatinization

Heating disrupts these cluster structures and double helices that is difficult to digest. For example, rice grains expand and soften when cooked because starch granules burst. Bomba rice or short-grain rice in Spanish has fewer amylose (15-20%) and absorb water more easily when cooked, so they have a thicker texture that is perfect for soaking up soups and sauces, like in paella.

Retrogradation

Retrogradation is both beneficial and detrimental in food. Amylose will form a very elastic hydrogel suitable for soft candies. Amylopectin is a soft, amorphous gel in aqueous medium. It is suitable for thick sauces and canned meat products.

On the other hand, as the starch molecules realign, the product becomes firmer and may expel water. Sometimes it is soggy in food surface. This not only damages food appearance, but also tends to feed microorganisms. Bread or popcorn becomes chewy during long-term storage is also attribute to double helix formed by amylopectin.

Some of the resistant starch in your diet comes from these chewy, starchy foods. Since not broken down in the small intestine easily, it is beneficial to control glucose absorption and blood sugar levels. In the large intestine, resistant starch is fermented by probiotics into healthful short-chain fatty acids.

Solubility of Amylose and Amylopectin?

Amylose is located in loosely bound amorphous regions that is near the granule surface. Heating causes them to first enter aqueous solution. Amylopectin forms a semi-crystalline structure. Branches provides more contact points to raise intermolecular forces. Large molecular weight also prevents them from being dragged into aqueous medium by water molecules. This means the more amylopectin the higher gelatinization temperature. The exception is glutinous rice containing almost no amylose. The reason is that some amylose is filled in semi-crystalline area to connect amylopectin clusters for stability.